CyToxiLux® , GranToxiLux®, and PanToxiLux?
Live, Single Cell-based Fluorogenic Cytotoxicity Assay Assay Kits
Principle of assays: Following the successful delivery of a lethal hit by cytotoxic lymphocytes, protease activities leading to cell death in individual target cells can be measured by flow cytometry or fluorescence microscopy. In each kit cleavage of a cell permeable fluorogenic substrate reports the following activities:
Measurements with CyToxiLux®, GranToxiLux®, and PanToxiLuxTM are superior to bulk assays e.g., LDH and 51Cr release, in terms of both time (0.3-2.0 hr. vs. 4 hr.) and sensitivity (relatively weak CTL responses against subdominant epitopes are detectable).
Detailed CyToxiLux® PLUS! instructions are available for download in PDF format Detailed GranToxiLux® PLUS! instructions are available for download in PDF format Detailed PanToxiLux® instructions are available for download in PDF format Detailed PanToxiLux? P2D2 instructions are available for download in PDF format